Fiber-optic glucose biosensor based on glucose oxidase (GOD) immobilised in a silica gel matrix

نویسندگان

  • M. Portaccio
  • M. Lepore
  • B. Della Ventura
  • O. Stoilova
  • N. Manolova
  • I. Rashkov
  • D. G. Mita
چکیده

In this paper, we report results obtained from a biosensor comprised of a monolithic silica sol–gel entrapping glucose oxidase (GOD) as sensing element coupled to a fibre-optic transducer element. GOD intrinsic fluorescence spectra were analysed in the UV and visible region in presence of different glucose concentrations, and the corresponding calibration curves were obtained. In addition, time course measurements were acquired following the procedures presented in previous works [1, 2]. Physicochemical and biochemical characterizations of our sol–gel are also reported.

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تاریخ انتشار 2009